ASTM E579-2004(2009) 溶液中硫酸奎宁的荧光检测限值的标准试验方法

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【英文标准名称】:StandardTestMethodforLimitofDetectionofFluorescenceofQuinineSulfateinSolution
【原文标准名称】:溶液中硫酸奎宁的荧光检测限值的标准试验方法
【标准号】:ASTME579-2004(2009)
【标准状态】:现行
【国别】:美国
【发布日期】:2004
【实施或试行日期】:
【发布单位】:美国材料与试验协会(US-ASTM)
【起草单位】:E13.01
【标准类型】:(TestMethod)
【标准水平】:()
【中文主题词】:
【英文主题词】:fluorescencespectrometers;molecularluminescence;molecularspectroscopy;Fluorescencespectrochemicalanalysis;Instrumentalmeasurement--spectrochemical;Limitofdetection(LOD);Minimumdetectableconcentration(MDC);Performance--laboratoryinstrum
【摘要】:Whendeterminingthelimitingdetectableconcentrationofafluorescentsubstance,itisusuallynecessarytoincreasethereadoutscaleofaphotoelectricinstrumenttoapointwherenoise(thatis,randomfluctuationsofthesystem)becomesapparent.Thisnoisewillbesuperimposeduponthesignalfromthesample.Inmolecularfluorescencespectroscopy,thelimitofdetectionforthesamplewillbedeterminedbythelimitingsignal-to-noiseratio,S/N,wherethesignal,S,isthedifferencebetweenreadingsobtainedwiththesampleandblanksolutions,andNisthetotalroot-mean-square(rms)noise.Thelimitofdetectionforthesamplewillbegivenbytheinstrumentreadingsthatgiveasignalequaltothreetimesthermsvalueofthenoise.Note28212;Factorsotherthannoiseaffectingthesampleconcentrationcorrespondingtothelimitofdetectioninclude:thespectralbandwidthsoftheexcitationandemissionmonochromators,theintensityoftheexcitinglightthatcanbeconcentratedonthesample,thefractionofthefluorescencecollectedbythedetectionsystem,theresponsetimeofthedetectionsystem,andthepurityofthesolvent.Thesizeandarrangementofthesamplecontainerwithrespecttothelightbeamsarealsoimportant,astheyaffectboththedesiredsignalandtheextraneoussignalthatonlycontributesnoise.1.1Thistestmethodemploysthesignal-to-noiseratiotodeterminethesensitivityofafluorescencemeasuringsystemintestingforthelimitofdetection(LOD)ofquininesulfatedihydrateinsolution.Theresultsobtainedwithquininesulfatedihydrateinsolutionaresuitableforspecifyinginstrumentperformanceonsampleshavingexcitationandfluorescencebandswiderthan10nmatornearroomtemperature.1.1.1Thistestmethodisnotintendedtobeusedas(1)arigoroustestofperformanceofinstrumentation,or(2),tointercomparethequantitativeperformanceofinstrumentsofdifferentdesign.IntercomparisonoftheLODbetweeninstrumentsiscommonlyexpressedastheratioofthewaterRamanpeakintensitytotheroot-mean-square(rms)noiseasmeasuredonafluorometerusinganexcitationwavelengthof350nmThistestmethodusestheexcitationandemissionpeakwavelengthsforquininesulfatedihydrateinsolution,whichareapproximately350nmand450nm,respectively..1.2Thistestmethodhasbeenappliedtofluorescence-measuringsystemsutilizingnon-laser,low-energyexcitationsources.Thereisnoassurancethatextremelyintenseilluminationwillnotcausephotodecompositionofthecompoundsuggestedinthistestmethod.Forthisreason,itisrecommendedthatthistestmethodnotbeindiscriminatelyemployedwithhighintensitylightsources.Thistestmethodisnotintendedtodetermineminimumdetectableamountsofothermaterials.Ifthistestmethodisextendedtoemployotherchemicalsubstances,theusershouldbeawareofthepossibilitythattheseothersubstancesmayundergodecompositionoradsorptionontocontainers.1.3AtypicalLODforconventionalfluorometersusingthistestmethodis1ngofquininesulfatepermL.1.4Thesuggestedshelflifeofa1mg/mLstocksolutionofquininesulfatedihydrateisthreemonths,whenstoredinthedarkinastopperedglassbottle.1.5ThevaluesstatedinSIunitsaretoberegardedasstandard.Nootherunitsofmeasurementareincludedinthisstandard.1.6Thisstandarddoesnotpurporttoaddressallofthesafetyproblems,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappropriates......
【中国标准分类号】:G15
【国际标准分类号】:
【页数】:3P.;A4
【正文语种】:英语